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Abstract Background Hypertrophic scar (HS), a fibroproliferative disorder caused by aberrant wound healing following skin injuries such as burns, lacerations and surgery, is characterized by invasive proliferation of fibroblasts and excessive extracellular matrix (ECM) accumulation. The dysregulation of autophagy is the pathological basis of HS formation. Previously, angiopoietin-2 (ANGPT2) was found to be overexpressed in HS fibroblasts (HSFs) compared with normal skin fibroblasts. However, whether ANGPT2 participates in the process of HS formation and the potential molecular mechanisms are not clear. Objective This study is intended to figure out the role of ANGPT2 and ANGPT2-mediated autophagy during the development of HS. Methods RT-qPCR was used to detect ANGPT2 expression in HS tissues and HSFs. HSFs were transfected with sh-ANGPT2 to knock down ANGPT2 expression and then treated with MHT1485, the mTOR agonist. The effects of sh-ANGPT2 or MHT1485 on the proliferation, migration, autophagy and ECM accumulation of HSFs were evaluated by CCK-8 assay, Transwell assay and western blotting. The expression of PI3K/Akt/mTOR pathway-related molecules (p-PI3K, p-Akt and p-mTOR) was assessed by western blotting. Results ANGPT2 expression was markedly upregulated in HS tissues and HSFs. ANGPT2 knockdown decreased the expression of p-PI3K, p-Akt and p-mTOR. ANGPT2 knockdown activated autophagy and inhibited the proliferation, migration, and ECM accumulation of HSFs. Additionally, the treatment of MHT1485, the mTOR agonist, on ANGPT2-downregulated HSFs, partially reversed the influence of ANGPT2 knockdown on HSFs. Study limitations The study lacks the establishment of more stable in vivo animal models of HS for investigating the effects of ANGPT2 on HS formation in experimental animals. Conclusions ANGPT2 downregulation represses growth, migration, and ECM accumulation of HSFs via autophagy activation by suppressing the PI3K/Akt/mTOR pathway. Our study provides a novel potential therapeutic target for HS.
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Objective@#To analyze the relationship between the toilet design and sanitary condition of primary and secondary schools in Chengdu and the epidemic situation of Norovirus infection, so as to provide scientific references for school toilet design and Norovirus prevention and control.@*Methods@#A total of 78 cases of norovirus epidemics from 2019 to 2020 were included in this study. These epidemics happened in 78 schools and the causes were indicated as human-to-human transmission. The Chi square and Mann-Whitney U test were used to compare categorical and continuous data respectively. Unconditional binary Logistic regression was used to examine the multivariate associations.@*Results@#Most epidemics happened from October in 2019 to March in 2020(79.49%, 62 cases), in primary schools (71.79%, 56 cases) and in the center area (52.56%, 41 cases). The median of the case number in each epidemic was 10. Overall, 56 schools (71.79%) were equipped with toilets which were flushed independently, and 22 schools (28.21%) were equipped with toilets which were flushed uniformly. There were 27 schools (34.62%) that did not have enough water taps. Logistic regression analysis found that those with toilets flushed uniformly had a stronger epidemic, compared to the schools with toilets flushed independently( OR=5.53, 95%CI=1.63-18.76, P <0.05).@*Conclusion@#In order to prevent intestinal infectious diseases , it is suggested that schools should design or reconstruct independent flushing toilets with adequate faucets.
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OBJECTIVES@#To study the correlation between 25-hydroxyvitamin D [25-(OH)D] and nephroblastoma in children and its value in assessing the prognosis of the disease.@*METHODS@#A total of 50 children with nephroblastoma who were admitted from January 2018 to December 2022 were included as the nephroblastoma group, and according to the postoperative pathological type, they were divided into a good prognosis group with 38 children and a poor prognosis group with 12 children. A total of 50 healthy children who underwent physical examination during the same period of time served as the healthy control group. The above groups were compared in terms of serum creatinine and 25-(OH)D level. A Spearman correlation analysis was used to investigate the correlation between serum 25-(OH)D level and therapeutic effect reaction. A multivariate logistic regression analysis was used to identify the risk factors affecting the prognosis of nephroblastoma in children.@*RESULTS@#The nephroblastoma group had significantly lower levels of serum creatinine and 25-(OH)D than the healthy control group (P<0.05). Compared with the good prognosis group, the poor prognosis group had a significantly larger tumor diameter, a significantly higher proportion of children with stage III-IV tumors, a significantly higher rate of tumor metastasis, and significantly lower serum levels of creatinine and 25-(OH)D (P<0.05). The Spearman correlation analysis showed that serum 25-(OH)D level was negatively correlated with therapeutic effect reaction (rs=-0.685, P<0.001). The multivariate logistic regression analysis showed that tumor diameter ≥10 cm, stage III-IV tumors, presence of tumor metastasis, and 25-(OH)D <19 ng/mL were closely associated with the poor prognosis of nephroblastoma in children (P<0.05). Serum 25-(OH)D level had an area under the curve of 0.805 (95%CI: 0.706-0.903, P<0.001) in evaluating the prognosis of nephroblastoma in children, with a Youden index of 0.512, a sensitivity of 0.938, and a specificity of 0.575 at the optimal cut-off value of 1.764 ng/mL.@*CONCLUSIONS@#There is a significant correlation between 25-(OH)D level and the prognosis of nephroblastoma in children, and 25-(OH)D can be used for prognosis prediction.
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Humans , Child , Creatinine , Vitamin D Deficiency/complications , Vitamin D , Calcifediol , Prognosis , Wilms Tumor , Kidney Neoplasms/complicationsABSTRACT
ABSTRACT Introduction Basketball is a sport with strong rivalry, being, therefore, a modality with great facility to cause sports injuries along its practice. Objective Investigate the status of sports injuries in college basketball for amateur students. Methods 480 college students who were not basketball experts were selected as research volunteers. The current status of sports injuries in the 12-week optional basketball course was investigated, and the main risk factors were analyzed. Results Among amateur male college students, the average playing age of third-grade male college students is higher than that of second-grade male college students, and the weekly playing frequency is lower than that of second-grade male college students. The incidence of basketball sports injuries among male college students who do not specialize in basketball from childhood to adulthood is up to 90.7%, and 85.5% during their college years. Conclusion In basketball sports, the most vulnerable parts of amateur male college students are the wrist joint, ankle joint, knee joint, leg, head and face, shoulder, waist, and back, respectively. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.
RESUMO Introdução O basquetebol é um esporte com forte rivalidade, sendo por isso uma modalidade com muita facilidade de causar lesões esportivas ao longo de sua prática. Objetivo Investigar a situação das lesões esportivas no basquetebol universitário para estudantes amadores. Métodos 480 estudantes universitários que não eram especializados em basquetebol foram selecionados como voluntários de pesquisa, e a situação atual das lesões esportivas no curso opcional de basquetebol de 12 semanas foi investigada, onde os principais fatores de risco foram analisados. Resultados Entre os estudantes universitários masculinos amadores, a idade média de jogo dos estudantes universitários masculinos da terceira série é maior do que a da segunda série, e a frequência semanal de jogo é menor do que a dos estudantes universitários masculinos da segunda série. A incidência de lesões esportivas de basquetebol entre os estudantes universitários masculinos que não são especializados em basquetebol desde a infância até a idade adulta é de até 90,7%, e 85,5% durante seus anos universitários. Conclusão No processo dos esportes de basquetebol, as partes mais vulneráveis dos estudantes universitários masculinos amadores são a articulação do pulso, articulação do tornozelo, articulação do joelho, perna, cabeça e rosto, ombro, cintura e costas, respectivamente. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.
RESUMEN Introducción El baloncesto es un deporte de fuerte rivalidad, siendo por tanto una modalidad con gran facilidad para provocar lesiones deportivas a lo largo de su práctica. Objetivo Investigar la situación de las lesiones deportivas en el baloncesto universitario para estudiantes aficionados. Métodos Se seleccionaron 480 estudiantes universitarios no especializados en baloncesto como voluntarios para la investigación, y se investigó la situación actual de las lesiones deportivas en el curso optativo de baloncesto de 12 semanas, donde se analizaron los principales factores de riesgo. Resultados Entre los estudiantes universitarios aficionados de sexo masculino, la edad media de juego de los estudiantes universitarios de tercer grado es superior a la de los estudiantes universitarios de segundo grado, y la frecuencia semanal de juego es inferior a la de los estudiantes universitarios de segundo grado. La incidencia de las lesiones deportivas de baloncesto entre los estudiantes universitarios de sexo masculino no especializados en baloncesto desde la infancia hasta la edad adulta es de hasta el 90,7%, y del 85,5% durante sus años universitarios. Conclusión En el proceso de los deportes de baloncesto, las partes más vulnerables de los estudiantes universitarios aficionados varones son la articulación de la muñeca, la articulación del tobillo, la articulación de la rodilla, la pierna, la cabeza y la cara, el hombro, la cintura y la espalda, respectivamente. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.
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Context: The safety and efficacy of irreversible electroporation (IRE) for locally advanced pancreatic carcinoma (LAPC) are well established. However, whether adjuvant chemoradiotherapy after IRE increases, the survival rate remains unknown. Therefore, this study evaluated the effect of chemoradiotherapy combined with IRE in patients with LAPC. Subjects and Methods: We retrospectively analyzed 42 patients with LAPC between July 2015 and December 2016 at PLA General Hospital treated with IRE or IRE combined with radiation and/or chemotherapy. These patients were divided into the IRE group and the combined-therapy group. All patients underwent computed tomography (CT), magnetic resonance imaging, and positron-emission tomography-CT and no signs of metastases were found. The prognosis of these patients was observed. Results: The times after operation and after diagnosis in the combined-therapy group (304.20 ± 118.54) and (334.40 ± 114.07) days, respectively, were better those than in the IRE group (214.36 ± 95.68) and (244.68 ± 110.61) days, respectively. Moreover, patients in the combined-therapy group had a significantly better survival rate than the IRE group (80 vs. 45.45%, P < 0.05). Conclusions: IRE combined with radiotherapy or chemotherapy was superior to IRE alone for the treatment of LAPC, as it prolonged the survival time and improved the survival rate, making it worthy of wide dissemination and clinical application
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PURPOSE@#Urosepsis in adults comprises approximately 25% of all sepsis cases, and is due to complicated urinary tract infections in most cases. However, its mechanism is not fully clarified. Urosepsis is a very complicated disease with no effective strategy for early diagnosis and treatment. This study aimed to identify possible target-related proteins involved in urosepsis using proteomics and establish possible networks using bioinformatics.@*METHODS@#Fifty patients admitted to the Urology Unit of Lanzhou General PLA (Lanzhou, China), from October 2012 to October 2015, were enrolled in this study. The patients were further divided into shock and matched-pair non-shock groups. 2-DE technique, mass spectrometry and database search were used to detect differentially expressed proteins in serum from the two groups.@*RESULTS@#Six proteins were found at higher levels in the shock group compared with non-shock individuals, including serum amyloid A-1 protein (SAA1), apolipoprotein L1 (APOL1), ceruloplasmin (CP), haptoglobin (HP), antithrombin-III (SERPINC1) and prothrombin (F2), while three proteins showed lower levels, including serotransferrin (TF), transthyretin (TTR) and alpha-2-macroglobulin (A2M).@*CONCLUSION@#Nine proteins were differentially expressed between uroseptic patients (non-shock groups) and severe uroseptic patients (shock groups), compared with non-shock groups, serum SAA1, APOL1,CP, HP, SERPINC1and F2 at higher levels, while TF, TTR and A2M at lower levels in shock groups.these proteins were mainly involved in platelet activation, signaling and aggregation, acute phase protein pathway, lipid homeostasis, and iron ion transport, deserve further research as potential candidates for early diagnosis and treatment. (The conclusion seems too simple and vague, please re-write it. You may focus at what proteins have been expressed and introduce more detail about its significance.).
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Adult , Aged , Female , Humans , Male , Middle Aged , Antithrombin III , Apolipoprotein L1 , Blood , Ceruloplasmin , Haptoglobins , Prealbumin , Pregnancy-Associated alpha 2-Macroglobulins , Proteomics , Prothrombin , Sepsis , Blood , Diagnosis , Genetics , Serum Amyloid A Protein , Transferrin , Urinary Tract InfectionsABSTRACT
Circular RNAs (circRNAs) from back-splicing of exon(s) have been recently identified to be broadly expressed in eukaryotes, in tissue- and species-specific manners. Although functions of most circRNAs remain elusive, some circRNAs are shown to be functional in gene expression regulation and potentially relate to diseases. Due to their stability, circRNAs can also be used as biomarkers for diagnosis. Profiling circRNAs by integrating their expression among different samples thus provides molecular basis for further functional study of circRNAs and their potential application in clinic. Here, we report CIRCpedia v2, an updated database for comprehensive circRNA annotation from over 180 RNA-seq datasets across six different species. This atlas allows users to search, browse, and download circRNAs with expression features in various cell types/tissues, including disease samples. In addition, the updated database incorporates conservation analysis of circRNAs between humans and mice. Finally, the web interface also contains computational tools to compare circRNA expression among samples. CIRCpedia v2 is accessible at http://www.picb.ac.cn/rnomics/circpedia.
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Animals , Humans , Mice , Databases, Genetic , Gene Expression Regulation , Internet , Molecular Sequence Annotation , RNA , Genetics , User-Computer InterfaceABSTRACT
Objective:To investigate The relationship between the disease progression of primary gastric cancer and preoperative neutrophil-to-lymphocyte ratio、platelet-to-lymphocyte ratio.Methods:A retrospective analysis of the clinical data of 188 cases treated in our hospital from April 2015 to February 2015 with primary gastric cancer.according to the peripheral venous blood NLR、PLR,analyze the relationship of NLR、PLR with different tumor infiltration depth、lymph node staging and TNM staging with statistical methods.Results:There is statistically significance of NLR、PLR between different groups (P＜0.01);tumor infiltration depth,lymph node staging and TNM stage of high NLR、PLR group in patients with gastric cancer were higher than low NLR、PLR group,the difference is statistically significant (P＜0.01);NLR is significantly positive correlated to PLR (r=0.379,P＜0.001);Multiple linear regression analysis shows the above factors had significant effects on preoperative NLR,PLR (P＜0.05).Conclusions:Preoperative peripheral blood NLR and PLR levels may be value in predicting the progression of gastric cancer,High preoperative NLR indicates later disease progression and worse prognosis.
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Objective:To investigate The relationship between the disease progression of primary gastric cancer and preoperative neutrophil-to-lymphocyte ratio、platelet-to-lymphocyte ratio.Methods:A retrospective analysis of the clinical data of 188 cases treated in our hospital from April 2015 to February 2015 with primary gastric cancer.according to the peripheral venous blood NLR、PLR,analyze the relationship of NLR、PLR with different tumor infiltration depth、lymph node staging and TNM staging with statistical methods.Results:There is statistically significance of NLR、PLR between different groups (P＜0.01);tumor infiltration depth,lymph node staging and TNM stage of high NLR、PLR group in patients with gastric cancer were higher than low NLR、PLR group,the difference is statistically significant (P＜0.01);NLR is significantly positive correlated to PLR (r=0.379,P＜0.001);Multiple linear regression analysis shows the above factors had significant effects on preoperative NLR,PLR (P＜0.05).Conclusions:Preoperative peripheral blood NLR and PLR levels may be value in predicting the progression of gastric cancer,High preoperative NLR indicates later disease progression and worse prognosis.
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<p><b>OBJECTIVE</b>To explore the expressions of SIgA and alpha l-AR in benign prostatic hyperplasia (BPH) complicated by chronic prostatitis (CP) and their implications.</p><p><b>METHODS</b>According to the preoperative findings of expressed prostatic secretion (EPS), transrectal prostate ultrasonography, prostate-specific antigen (PSA), international prostate symptom score (IPSS), clinical symptoms, chronic pelvic pain syndrome (CPPS) and postoperative histopathology, 62 cases of BPH pathologically confirmed after transurethral plasma kinetic resection of the prostate (PKRP) were divided into a BPH group (n = 32) and a BPH + CP group (n = 30). The expressions of SIgA and alpha 1-AR in the prostate tissue were determined by immunohistochemistry and PT-PCR.</p><p><b>RESULTS</b>Of the 62 cases, 30 were found to be BPH + CP, and the other 32 to be BPH. The expressions of SIgA and alpha1-AR were significantly higher in the BPH + CP than in the BPH group (0.380 8 +/- 0.144 3 vs 0.295 4 +/- 0.008 4 and 0.440 5 +/- 0.104 1 vs 0.383 2 +/- 0.013 6, P < 0.05).</p><p><b>CONCLUSION</b>The upregulated expressions of SIgA and alpha1-AR expression in BPH complicated by CP suggest a certain association between CP and BPH, and that inflammation may be a pathogenic factor of BPH and correlate with its pathological development.</p>
Subject(s)
Aged , Humans , Male , Middle Aged , Chronic Disease , Immunoglobulin A, Secretory , Metabolism , Prostate , Metabolism , Pathology , Prostatic Hyperplasia , Metabolism , Pathology , Prostatitis , Metabolism , Pathology , Receptors, Androgen , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To investigate the clinical effect of electrochemical therapy (ECT) combined with pingyangmycin (PYM) injection for the treatment of high-return flow venous malformation.</p><p><b>METHODS</b>A total of 68 cases of the high-return flow venous malformation were retrospectively analyzed, in which 32 cases received simplely ECT,and 36 cases received ECT combined with PYM injection. The patients were followed up from 6 to 30 months.</p><p><b>RESULTS</b>The effectiveness of ECT was 78% (25/32), and of ECT combined with PYM injection was 97% (35/36). The effectiveness of ECT combined with PYM injection was higher than that of ECT (P < 0.05). No serious adverse effects were encountered.</p><p><b>CONCLUSIONS</b>ECT combined with PYM injection is an effective method for the treatment of high-return flow venous malformation.</p>
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Adolescent , Adult , Aged , Child , Child, Preschool , Female , Humans , Male , Middle Aged , Young Adult , Bleomycin , Therapeutic Uses , Blood Flow Velocity , Combined Modality Therapy , Electrochemical Techniques , Methods , Follow-Up Studies , Injections , Retrospective Studies , Vascular Malformations , Drug Therapy , Therapeutics , Veins , Congenital AbnormalitiesABSTRACT
Introduction:The 2010 targets of the China Hepatitis B Prevention Programme were a prevalence of hepatitis B surface antigen (HBsAg) less than 1.0% for children less than five years old and less than 6.0% for the total population. This survey assessed the prevalence of Hepatitis B infection in Lianyungang, Jiangsu province, China in 2009–2010.Methods:Multistage sampling was used with 2372 subjects among 17 selected villages. Blood specimen collection and testing by enzyme-linked immunosorbnet assay (ELISA) were completed using the following markers for hepatitis infection: HBsAg and antibody to HBsAg (anti-HBs); hepatitis B e antigen (HBeAg) and antibody to HBeAg (anti-HBe); and hepatitis B core antibody (total anti-HBc). The data were analysed with Epi Info, version 3.3.2.Results:The prevalence of HBsAg was 2.4% (95% Confidence Interval [CI]: 1.8–3.0; Adjusted Prevalence [AP] 2.9%); anti-HBs prevalence was 51.1% (95% CI: 49.1–53.1; AP 49.2%) and total anti-HBc prevalence was 41.7% (95% CI: 39.8–43.7; AP 45.5%). The prevalence of HBsAg and total anti-HBc positivity increased from young to older age groups, yet the prevalence of anti-HBs positivity decreased from young to older age groups (PP= 0.108 and 0.089), but females had a higher prevalence than males for total anti-HBc positivity (P< 0.001). Discussion: This survey showed that in 2010 the prevalence of HBsAg among children aged less than five years was lower than the national target of 1.0% and that the prevalence of HBsAg for the total population was lower than the national target of 6.0%.
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<p><b>OBJECTIVE</b>To explore the therapeutic effect of electrochemical therapy(ECT) for high-flow vascular malformation in maxillofacial region.</p><p><b>METHODS</b>38 patients with high-flow vascular malformation in maxillofacial region were treated with ECT. The voltage was 6-8 V, and the electric current was 80-100 mA. The electric quantity was 10-20 C/cm2.</p><p><b>RESULTS</b>Among the 38 cases, 32 cases received only one treatment, 5 cases received two treatments and one case received three treatments. The patients were followed up for 2 months to 3 years. The total effective rate was 100% with 55.26% of cure rate and 44.74% of partial cure rate. No hemolysis happened during follow-up period.</p><p><b>CONCLUSIONS</b>ECT is a simple and effective method for high-flow vascular malformation with less morbidity.</p>
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Adolescent , Adult , Child , Child, Preschool , Female , Humans , Male , Middle Aged , Young Adult , Electrochemotherapy , Facial Neoplasms , Drug Therapy , Follow-Up Studies , Hemangioma , Drug Therapy , Treatment OutcomeABSTRACT
<p><b>OBJECTIVE</b>To investigate the classification of alar base depression, so as to provide the reference for the surgical management of secondary nasal deformity of unilateral cleft lip.</p><p><b>METHODS</b>From Jul. 2008 to Feb. 2009, 26 cases with secondary deformity of unilateral cleft lip were treated. All the patients underwent 3-dimensional CT for maxillary measurement. The nasal soft tissue measurement was performed pre- and post-operatively. The relationship between the maxillary and soft tissue at alar base was analyzed. The nasal deformity was classified.</p><p><b>RESULTS</b>The location of alar base was not related to the form of piriform aperture, but the bony defect at the alar base was correlated to the patient satisfactory. The nasal deformity was graded as I when the depression at alar base was less than 4.5 mm in depth, as II when it was 4.5-5.0 mm in depth, and as III when it was more than 5 mm in depth. The deformity could be corrected with only soft tissue plasty for grade I, with soft tissue plasty or artificial implants for grade II, with combined bone autograft or alveolar cleft repair for grade III.</p><p><b>CONCLUSIONS</b>The depression at maxillary does not necessarily result in alar base depression. The alar base can be adjust to proper position through operation. The operation should be designed based on the preoperative nasal measurement.</p>
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Adolescent , Adult , Child , Female , Humans , Male , Young Adult , Cleft Lip , General Surgery , Nose , Congenital Abnormalities , General Surgery , Postoperative Complications , General Surgery , Rhinoplasty , Methods , Treatment OutcomeABSTRACT
<p><b>OBJECTIVE</b>To study the therapeutic effect of one-staged repair of unilateral cleft lip an nasal deformity with modified Mohler.</p><p><b>METHODS</b>The unilateral cleft lip and nasal deformity were repaired in one stage with modified Mohler. The nasal retainers were kept for at least 6 months. The patients were followed up at 3 months and 12 months after operation.</p><p><b>RESULTS</b>From Sept. 1998 to Aug. 2008, 103 cases, aged from 2.5 m to 12 m, were treated. 3 months after operation, 97 of 103 patients had good appearance of both lips and noses, which included unobvious scars, symmetric Cupid's bows and nostrils, intermedial nasal columellae and eminentia nasal tips. 6 of them had good appearance of noses only with a little malposition of Cupid' s bows. 89 patients were followed up for 12 months with no deterioration of nasal deformity and delayed growth of greater alar cartilages. 3 patients who had malposition of Cupid's bows also got better.</p><p><b>CONCLUSIONS</b>The one-staged repair of unilateral cleft lip and nasal deformity with modified Mohler is very practical.</p>
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Female , Humans , Infant , Male , Cleft Lip , General Surgery , Nose , Congenital Abnormalities , Plastic Surgery Procedures , Classification , MethodsABSTRACT
The roles of Indian hedgehog (Ihh) signaling pathway in the proliferation and apoptosis of precartilaginous stem cells (PSCs) were investigated.PSCs,labeled with fibroblast growth factor receptor 3 (FGFR-3),were isolated from neonatal rats by immanomagnetic separation.After identifi-cation with FGFR-3 and Col Ⅱ,the cells were incubated with different concentrations of cyclopamine (cyclo),the specific inhibitor of lhh signaling pathway.The morphologic changes of the cells were observed under the inverted phase contrast microscope.The mRNA expression levels of Ibh,para-thyroid hormonerelated peptide (PTHrP),protein Patched (Ptch),Bcl-2 and p21 were detected by RT-PCR.The protein expression levels of Ihh and Ptch were measured by Western blot.MTT assay was used to examine the effects of cyclo on proliferation of PSCs.Apoptosis rate of PSCs was exam-ined by Annexin V/PI assay of flow cytometric analyses.After PSCs were incubated with cyclo,ob-vious morphologic changes were observed as compared with the control group.The mRNA expres-sion levels of PTHrP,Ptch and Bcl-2 were decreased to varying degrees in a cyclo dose-dependent manner.However,the expression levels of lhh and p21 mRNA were increased.The protein expres-sion of Ptch and Ihh had the same change as the mRNA expression.Meanwhile,cyclo could obvi-ously inhibit the proliferation and promote the apoptosis of PSCs.The results indicated that Ihh sig-naling pathway plays an important role in regulating the proliferation and apoptosis of PSCs,which is probably mediated by Bcl-2 and p21.
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<p><b>OBJECTIVE</b>To investigate the expression and correlation of mast cell, Clusterin/apoJ and transforming growth factor-beta (TGF-beta) in the different stages of human dermal hemangioma.</p><p><b>METHODS</b>Immunohistochemical stain technique (SABC) and toluidine blue (TB) stain technique were respectively used to detect the expression level of Clusterin/apoJ and TGF-beta and the number of mast cells in the different stages of human dermal hemangioma.</p><p><b>RESULTS</b>There was remarkable statistical difference between the advanced stage of proliferative hemangioma and the other stages of proliferative hemangioma in the number of mast cell(P<0.01). There was also remarkable statistical difference between the early stage of involutional hemangioma and the other stages of involutional hemangioma in the number of mast cell (P<0.01). The expression of Clusterin/apoJ and TGF-beta in the advanced stage of proliferative hemangioma was significantly higher than the other stages in proliferative hemangioma (P<0.01). The expression of Clusterin/apoJ and TGF-beta in the early stage of involutional hemangioma was significantly higher than the other stages in involutional hemangioma (P<0.01). There was a significantly positive correlation between Clusterin/apoJ and TGF-beta in the different stages of human dermal hemangioma (P<0.01). The expression level of Clusterin/apoJ and TGF-beta was positively correlated with the number of mast cell in the different stages of human dermal hemangioma (P<0.01).</p><p><b>CONCLUSION</b>Mast cell may play a promotive role of apoptosis during the spontaneous regulate the expression of Clusterin/apoJ and promote the spontaneous involution of human dermal hemangioma.</p>
Subject(s)
Humans , Apoptosis , Clusterin , Glycoproteins , Hemangioma , Mast Cells , Molecular Chaperones , Transforming Growth Factor betaABSTRACT
It has been suggested that progression of bladder transitional cell cancer (BTCC) may be regulated at the molecular level by a typical pattern of expression of genes involved in apoptosis. Re- cently Livin, belonging to the inhibitors of apoptosis (IAP) family, has been found to be expressed in most solid tumors, where its expression is suggested to have clinical significance. In order to explore the significance of Livin expression in the development of BTCC, immunohistochemistry and RT-QPCR were used to detect the expression of Livin mRNA in tumor tissues and adjacent normal tissues of 30 cases of BTCC. The results showed that the positive rate of Livin expression in adjacent normal tissues and tumor tissues was 0 and 60% (18/30) respectively. The -△△CT value of Livin in BTCC tissues was 8.0454 (7.4264-8.6644) times of that in adjacent normal tissues. The expression of Livin mRNA had no correlation with tumor pathological grades and clinical stages. It was sug- gested that there was weak expression ? Livin mRNA in adjacent normal tissues, but strong in tumor tissues.
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<p><b>OBJECTIVE</b>To evaluate the significance of cardiac output (CO) response against exercise determined by IGR method and LVEF, 6 MWT distance in patients with chronic heart failure (CHF).</p><p><b>METHOD</b>To adopt 6 MWT, and before and after the test measuring the CO by the IGR method, furthermore, measure LVEF to 36 patients (heart failure group) with CHF, compare with the health groups (control group).</p><p><b>RESULTS</b>The 6MWT distance of heart failure group (333.00 +/- 49.64) m decrease compared with the control group (582.56 +/- 67.97) m (P < 0.01), moreover, the distance of NYHA class III (314.82 +/- 36.27) m is significantly shorter than II (361.57 +/- 55.42) m (P < 0.05). The LVEF of heart failure group (47.0 +/- 0.4)% reduce compared with the control group (66.9 +/- 5.2)% (P < 0.01), and the data of NYHA class III (43.3 +/- 10.3)% is significantly lower than II (52.8 +/- 7.6)% (P < 0.01). The increase in CO response against exercise of heart failure group (5.97 +/- 1.89) L/min decrease compared with control group (8.88 +/- 0.52) L/min (P < 0.01), furthermore, the value of NYHA class III (5.31 +/- 1.52) L/min, compared with II (7.01 +/- 1.98)L/min, is obviously lower (P < 0.01). The 6MWT distance correlates positively with the increase in CO response against exercise (r = 0.63, P < 0.01), but the correlation is not found between the increase CO response against exercise and the LVEF (r = 0.11, P > 0.05).</p><p><b>CONCLUSION</b>Our results show that CO response against exercise measured by IGR method, with the advantages of being noninvasive, safe, convenient and accurate, combining with the 6MWT can evaluate cardiac reserve in patient with CHF.</p>